Description
A comprehensive and accessible survey of the best current accomplishments of GMO research in all their complexity and ramifications. The authors introduce the fundamentals of biotechnology as a scientific discipline, show how GMO research is conducted today, discuss the problems that have arisen from genetic technology and the tools needed to resolve them, and describes how GMO-derived technology may impact our lives in the future. On the technical side, the authors examine a wide range of current technologies employed for constructing GMOs, and describe approaches to novel research, appropriate protocols, and the process of constructing and screening a GMO. The discussion of plant and animal cells covers new strategies employed and the large-scale expression and purification of recombinant products in cultured cells. Social political, and legal issues are also discussed. Part I. Introduction Introduction Sarad R. Parekh and Anne Gregg Part II. Microbial GMOs Genetically Modified Microorganisms: Development and Applications Lei Han Producing Proteins Derived From Genetically Modified Organisms for Toxicology and Environmental Fate Assessment of Biopesticides Steven L. Evans Genetically Modified Organisms: Biosafety and Ethical Issues Douglas J. Stemke Part III. Mammalian GMOs Large-Scale Exogenous Protein Production in Higher Animal Cells William Whitford Biosafety, Ethics, and Regulation of Transgenic Animals Raymond Anthony and Paul B. Thompson Transgenic Aquatic Animals Sarad R. Parekh Part IV. Plant GMOs Development of Genetically Modified Agronomic Crops Manju Gupta and Raghav Ram Gene Silencing in Plants: Nature’s Defense W. Michael Ainley and Siva P. Kumpatla Value Creation and Capture With Transgenic Plants William F. Goure Biosafety Issues, Assessment, and Regulation of Genetically Modified Food Plants Yong Gao Conclusion and Future Directions Sarad R. Parekh Glossary Index
