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Preface… Table of Contents… Contributing Authors… Part 1 Engineering Mammalian Cells to Sense Biologically Relevant Inputs 1. Generation of CAR-T Cells by Lentiviral Transduction Atsushi Okuma 2. Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells Leo Scheller 3. Engineering Mammalian Cells to Control Glucose Homeostasis Jiawei Shao, Xinyuan Qiu, and Mingqi Xie 4. Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System Akihiro Eguchi and Masahiro Kawahara 5. Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs Hideyuki Nakanishi and Hirohide Saito Part 2 Engineering Mammalian Cells to Sense Artificial Inputs 6. Green Light-Controlled Gene Switch for Mammalian and Plant Cells Nils Schneider, Claire V. Chatelle, Rocio Ochoa-Fernandez, Matias D. Zurbriggen, and Wilfried Weber 7. Sonogenetic Modulation of Cellular Activities in Mammalian Cells Yao-Shen Huang, Ching-Hsiang Fan, Wei-Ting Yang, Chih-Kuang Yeh, and Yu-Chun Lin 8. Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells Yuanhuan Yu, Guiling Yu, and Haifeng Ye 9. Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice Guiling Yu, Yuanhuan Yu, and Haifeng Ye 10.Construction of Caffeine-Inducible Gene Switches in Mammalian Cells Daniel Bojar Part 3 Precise Genome Engineering Techniques using CRISPR-Cas Systems 11. Multiplexed Genome Engineering with Cas12a Niels R. Weisbach, Ab Meijs, and Randall J. Platt 12. Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells Soh Ishiguro and Nozomu Yachie 13. Optical Control of Genome Editing by Photoactivatable Cas9 Takahiro Otabe, Yuta Nihongaki, and Moritoshi Sato Part 4 Engineering Mammalian Cells in Combination with Chemical Compounds/Systems 14. Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT Sachio Suzuki, Yuka Hatano, Tatsuyuki Yoshii, and Shinya Tsukiji 15. Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules Hideki Nakamura 16.AgDD System: A Chemical Controllable Protein Aggregates in Cells Yusuke Miyazaki 17. Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme Yasunori Okamoto and Ryosuke Kojima 18.Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor Yuri Hayata, Ryosuke Ueki, and Shinsuke Sando Part 5 New Techniques to Engineer Specific Mammalian Cells in a Targeted Manner 19. Protocol for de novo Gene Targeting via In Utero Electroporation Yuji Tsunekawa, Raymond Kunikane Terhune, and Fumio Matsuzaki 20. Magnetically Guided Single Cell Virus Stamping Rajib Schubert Subject Index List…
