Description
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens. PART I: Fundamental Aspects And Freezing Technology 1 Principles of Cryopreservation David E. Pegg 2 Principles of Cryopreservation By Vitrification Gregory M. Fahy and Brian Wowk 3 Modeling and Optimization of Cryopreservation James Benson 4 The Principles of Freeze-Drying Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward PART II: Methods To Study Freezing and Drying Processes 5. Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells Willem F. Wolkers and Harritte Oldenhof 6. Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations Wendell Q. Sun 7. Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy Jens O. M. Karlsson 8. Laser Scanning Microscopy in Cryobiology Frank Stracke, Asger Kreiner-Mller, and Heiko Zimmermann 9. Low Temperature Electron Microscopy Roland A. Fleck PART III: Cryopreservation Protocols 10 Cryopreservation of Semen From Domestic Livestock Harald Sieme, Harritte Oldenhof 11. Cryopreservation of Mammalian Oocytes Victoria Keros, Barry J. Fuller 12. Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts Juergen Liebermann 13 Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification Julia C. Neubauer, Axel F. Beier, Nils Geijsen, and Heiko Zimmermann 14 Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. Mcgowan, and H. Robin Tervit 15 Membrane Modification Strategies for Cryopreservation Phillip H. Purdy and James K. Graham 16. Sperm Clean-Up and Density Centrifugation Procedures Harald Sieme and Harritte Oldenhof 17. Cryopreservation of Red Blood Cells Johan W. Lagerberg 18. Cord Blood Clinical Processing, Cryopreservation, and Storage Heidi Elmoazzen and Jelena L. Holovati 19. Directional Freezing For Large Volume Cryopreservation Joseph Saragusty 20 Vitrification Of Heart Valve Tissues Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell 21 Cryopreservation of Plant Cell Lines Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack 22 Writing Standard Operating Procedures (Sops) For Cryostorage Protocols: Using Shoot Meristem Cryopreservation As An Example Keith Harding and Erica E. Benson PART IV: Freeze-Drying Protocols 23 Freeze-Drying Of Proteins Baolin Liu, Xinli Zhou 24 Freeze-Drying Of Lactic Acid Bacteria Fernanda Fonseca, Stphanie Cenard, Stphanie Passot 25 Freeze-Drying Of Mammalian Sperm Levent Keskintepe, Ali Eroglu 26 Freeze-Drying Of Decellularized Heart Valve Tissues Willem F. Wolkers, Andres Hilfiker
