Description
Part I – Electron Cryotomography 1. Getting Started with in situ cryo-electron Tomography Daniel Serwas and Karen M. Davies 2. Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons Ryan K. Hylton, Victoria H. Seader, and Matthew T. Swulius 3. Practical Approaches for Cryo-FIB Milling and applications for Cellular Cryo-Electron Tomography Vinson Lam and Elizabeth Villa 4. In situ Imaging and Structure Determination of Biomolecular Complexes using Cryo-Electron Tomography Mohammed Kaplan, William J Nicolas, Wei Zhao, Stephen D Carter, Lauren Ann Metskas, Georges Chreifi, Debnath Ghosal, and Grant J. Jensen Part II- Single Particle Analysis and Electron Cryomicroscopy 5. Progress Towards CryoEM: Negative-stain Procedures for Biological Samples Shane Gonen 6. Setting up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection Mark A. Herzik, Jr. 7. Multi-body refinement of cryo-EM images in RELION Takanori Nakane and Sjors H.W. Scheres 8. Evaluating Local and Directional Resolution of Cryo-EM Density Maps Sriram Aiyer, Cheng Zhang, Philp R. Baldwin, and Dmitry Lyumkis 9. Automated modeling and Validation of Protein Complexes in cryo-EM Maps Tristan Cragnolini, Aaron Sweeney, and Maya Topf 10. 2D Electron Crystallography of Membrane Protein Single-, Double- and Multi-Layered Ordered Arrays Matthew C. Johnson, Yusuf M. Uddin, Kasahun Neselu, and Ingeborg Schmidt-Krey Part III-Electron Crystallography of 2d Crystals 11. Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction Ka-Yi Chan, Chloe Du Truong, Yu-Ping Poh, and Po-Lin Chiu 12. Single Particle Analysis for High Resolution 2D Electron Crystallography Ricardo Righetto and Henning Stahlberg 13. MicroED Sample Preparation and Data Collection for Protein Crystals Guanhong Bu and Brent L. Nannenga PART IV – MICROCRYSTAL ELECTRON DIFFRACTION (MICROED) 14. Detection of Microcrystals for CryoEM Simon Weiss, Sandra Vergara, Guowu Lin, and Guillermo Calero 15. Low-dose Data Collection and Radiation Damage in MicroED Johan Hattne 16. Automation of Continuous-rotation Data Collection for MicroED M. Jason de la Cruz 17. Ab initio Determination of Peptide Structures by MicroED Chih-Te Zee, Ambarneil Saha, Michael R. Sawaya, and Jose A. Rodriguez
